DeadEnd™ Colorimetric TUNEL System

TUNEL assay cell type - Islets of langerhans (Beta cells)

Experiment
TUNEL assay cell type - Islets of langerhans (Beta cells)
Product
DeadEnd™ Colorimetric TUNEL System from Promega
Manufacturer
Promega

Protocol tips

Protocol tips
Fix with 4% paraformaldehyde solution or 10% buffered formalin in PBS for 15 minutes at room temperature.

After rTdT reaction mix incubate the slides at 37°C for 60 minutes inside a humidified chamber

Publication protocol

Islets were incubated with 3 μmol/L calcein-AM (Life Technologies) and 2.5 μmol/L propidium iodide (Sigma-Aldrich) before detection of absorbance/emission at 491/525 nm and 561/620 nm, respectively. The islet area occupied by dead cells was expressed as a unitary ratio versus that occupied by live cells. Apoptosis was assessed using a TUNEL staining kit (Promega) according to the manufacturer’s instructions, and islets were counterstained against insulin before detection of absorbance/emission at 491/525 nm and 561/620 nm, respectively.

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Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - Islets of langerhans (Beta cells) using DeadEnd™ Colorimetric TUNEL System from Promega.

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Manufacturer protocol

Download the product protocol from Promega for DeadEnd™ Colorimetric TUNEL System below.

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