LC3B Antibody #2775

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Use 0.5 µg/ml for HRPO/ECL detection

Protocol tips
Use 0.5 µg/ml for HRPO/ECL detection

Publication protocol

Cells lysates (15–40 µg) were subjected to electrophoresis in 10% or 12% SDS-polyacrylamide gels. Gels were transferred to polyvinylidene difluoride membrane, blotted with primary and secondary antibodies as indicated, and detected by an enhanced chemiluminescence detection system (Western Lightning Plus-ECL; PerkinElmer, Waltham, MA). Scanning densitometry analysis was performed using Scion Image (Scion Corporation, Frederick, MD).

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Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Autophagy assay cell type - Mast cell using LC3B Antibody #2775 from Cell Signaling Technology.

Manufacturer protocol

Download the product protocol from Cell Signaling Technology for LC3B Antibody #2775 below.

Download manufacturer protocol

Videos

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