2x Laemmli Sample Buffer

Protein isolation Bacteria - Vibrio alginolyticus

Experiment
Protein isolation Bacteria - Vibrio alginolyticus
Product
2x Laemmli Sample Buffer from Bio-Rad Laboratories
Manufacturer
Bio-Rad Laboratories

Protocol tips

Protocol tips
- Follow the manufacturer's instructions.

Publication protocol

An equal volume of 2X Laemmli sample buffer (Bio-Rad) was added into each sample and all samples were boiled at 100°C for 5 min and loaded onto a 12% SDS-PAGE. After electrophoresis proteins were transferred to a PVDF membrane (Bio-Rad) and the membrane was blocked with 5% skimmed milk in PBS containing 0.05% (v/v) Tween 20 (PBS-T).

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Discussion

Discussion

5 years ago

Author: Hollie Fowler United Kingdom

How can I deal with my pellet being too viscous?

I am using 8M Urea to lyse my cells but after centrifugation my pellet is very viscous. This causes me trouble when I try to collect the supernatant. Is there any way to avoid it and do you think it will have an effect on the concentration of my protein?

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Papers

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Manufacturer protocol

Download the product protocol from Bio-Rad Laboratories for 2x Laemmli Sample Buffer below.

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