Osteopontin (human), ELISA kit

ELISA Human - OPN

Experiment
ELISA Human - OPN
Product
Osteopontin (human), ELISA kit from Enzo Life Sciences
Manufacturer
Enzo Life Sciences

Protocol tips

Upstream tips
-All reagents are stable as supplied at 4°C, except the Recombinant HO-1 Standard, which should be stored at -20°C. For optimum storage, the Recombinant HO-1 Standard should be aliquotted into smaller portions and stored at -20°C.
Protocol tips
-Include a standard curve each time the assay is performed.
-Mix all reagents and samples gently, yet thoroughly, prior to use. Avoid foaming of reagents.
-In this protocol, room temperature refers to 20-28C. The room temperature should remain within this range throughout the assay.
-Consistent, thorough washing of each well is critical.

Publication protocol

Osteopontin levels in glioma conditioned media were determined by ELISA (#ADI-900-142, ENZO Life Sciences).

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Papers

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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for Osteopontin (human), ELISA kit below.

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