TumorTACS™ In Situ Apoptosis Detection Kit

TUNEL assay cell type - Mouse liver tissue

Experiment
TUNEL assay cell type - Mouse liver tissue
Product
TumorTACS™ In Situ Apoptosis Detection Kit from Bio-Techne
Manufacturer
Bio-Techne

Protocol tips

Upstream tips
Dry overnight at room
temperature or for at least 2 hours at 45 oC on a slide warme
Protocol tips
Incubate 30 minutes at room temperature.

Add Labeling Reaction Mix and
incubate at 37 ˚C for 1 hour in a humidity chamber
Downstream tips
If no staining in TACS Nuclease, Increase incubation time with Proteinase K (up to maximum 60 minutes) or Cytonin (up to overnight at 4 C).

Publication protocol

The 5 μm tumor tissue sample sections were analyzed by TUNEL staining using a TumorTACS™ In Situ Apoptosis Detection kit in accordance with the manufacturer's instructions (R&D Systems, Inc., Minneapolis, MN, USA). The apoptotic diaminobenzidine (DAB)-positive cells (brown stained) were counted in five arbitrarily selected microscopic fields at a ×400 magnification (DM IL LED Microscope; Leica Microsystems GmbH, Wetzlar, Germany).

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Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - Mouse liver tissue using TumorTACS™ In Situ Apoptosis Detection Kit from Bio-Techne.

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Manufacturer protocol

Download the product protocol from Bio-Techne for TumorTACS™ In Situ Apoptosis Detection Kit below.

Download PDF Download manufacturer protocol

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