DeadEnd™ Colorimetric TUNEL System

TUNEL assay cell type - 3T3 L1 mouse adipose tissue

Experiment
TUNEL assay cell type - 3T3 L1 mouse adipose tissue
Product
DeadEnd™ Colorimetric TUNEL System from Promega
Manufacturer
Promega

Protocol tips

Downstream tips
- When there is a loss of tissue from slides, decrease
Proteinase K incubation time.

Publication protocol

Apoptosis in adipocytes was observed and detected by various methods, including caspase 3/7 activation analysis, TUNEL assay, and flow cytometry. Caspase 3/7 activity was quantitated by using a Caspase-Glo® 3/7 Assay Kit (Promega, CA, USA) according to the manufacturer's instructions. A DeadEnd™ Fluorometric TUNEL System (Promega) was used to observe and measure the adipocyte apoptosis. The number of apoptotic adipocytes was quantitated by flow cytometry assay (Gallios, Beckman Coulter, USA) with the Annexin V/PI double-staining method according to the manufacturer's instructions. (The Annexin V/PI apoptosis assay kit was purchased from Life Technologies, USA).

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Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - 3T3 L1 mouse adipose tissue using DeadEnd™ Colorimetric TUNEL System from Promega.

Paper title
Expression Pattern and Regulatory Role of microRNA-23a in Conjugated Linoleic Acids-Induced Apoptosis of Adipocytes.
Full paper
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Manufacturer protocol

Download the product protocol from Promega for DeadEnd™ Colorimetric TUNEL System below.

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