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differentiation, 2.5×105 cells were seeded into 15 ml polypropylene culture tubes. The cells were centrifuged at 150 × g for 5 min at room temperature. The caps of the tubes were loosened by one half turn to allow gas exchange, and the tubes were incubated at 37°C, in a humidified atmosphere of 5% CO2. |
After 24 h, freshly prepared Complete Chondrogenic Medium (Cyagen Biosciences, Inc.) was to each tube. |
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Upstream tips |
differentiation, 2.5×105 cells were seeded into 15 ml polypropylene culture tubes. The cells were centrifuged at 150 × g for 5 min at room temperature. The caps of the tubes were loosened by one half turn to allow gas exchange, and the tubes were incubated at 37°C, in a humidified atmosphere of 5% CO2. |
Protocol tips |
After 24 h, freshly prepared Complete Chondrogenic Medium (Cyagen Biosciences, Inc.) was to each tube. |
Publication protocol
After 24 h, freshly prepared Complete Chondrogenic Medium (Cyagen Biosciences, Inc.) was to each tube.
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