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194 mL human mesenchymal stem cell chondrogenic differentiation basal medium; 20 μL dexamethasone; 600 μL ascorbate; 2 mL insulin, transferrin, and selenium supplement; 200 μL sodium pyruvate; 200 μL proline; and 2 ml TGF-β3; Cyagen, Guangzhou, China) or incomplete chondrogenic induction medium lacking TGF-b3 |
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Protocol tips |
194 mL human mesenchymal stem cell chondrogenic differentiation basal medium; 20 μL dexamethasone; 600 μL ascorbate; 2 mL insulin, transferrin, and selenium supplement; 200 μL sodium pyruvate; 200 μL proline; and 2 ml TGF-β3; Cyagen, Guangzhou, China) or incomplete chondrogenic induction medium lacking TGF-b3 |
Publication protocol
194 mL human mesenchymal stem cell chondrogenic differentiation basal medium; 20 μL dexamethasone; 600 μL ascorbate; 2 mL insulin, transferrin, and selenium supplement; 200 μL sodium pyruvate; 200 μL proline; and 2 ml TGF-β3; Cyagen, Guangzhou, China) or incomplete chondrogenic induction medium lacking TGF-b3
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