APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma

Experiment
TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma
Product
APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
- During the assay, cells were treated at different time intervals: 24, 48, and 72 hours.
Protocol tips
- Fixed with 1% (w/v) paraformaldehyde in PBS for 15 min
Downstream tips
- If signal intensity is low, increase incubation time for the DNA-labeling reaction

Publication protocol

Apoptotic cells were detected using the APO-BrDU TUNEL Assay Kit (Invitrogen). A549 cells were treated at different time intervals: 24, 48, and 72 hours. Cells were harvested, washed, and fixed with 1% (w/v) paraformaldehyde. The cells were then centrifuged, washed, and fixed with ice-cold 70% ethanol. DNA labeling was performed according to the manufacturer's instructions, and the cells were analyzed using Accuri C6 flow cytometer.

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Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU from Thermo Fisher Scientific.

Paper title
Induction of Apoptosis and Cell Cycle Blockade by Helichrysetin in A549 Human Lung Adenocarcinoma Cells
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU below.

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