ApoAlert™ DNA Fragmentation Assay Kit

TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma

Experiment
TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma
Product
ApoAlert™ DNA Fragmentation Assay Kit from Takara Bio Inc
Manufacturer
Takara Bio Inc

Protocol tips

Upstream tips
- 1.5 × 106 cells were platted for culture.
Downstream tips
- No positive signal could be due to poor permeabilization with Triton X-100

Publication protocol

The cells (1.5 × 106) were treated with different concentrations of Sub-A for 48 h. DNA was extracted and separated by 2% agarose gel electrophoresis. The gel was stained with 0.5 mg/mL ethidium bromide (EtBr), placed on a UV transilluminator, and then photographed.

Quantitative analysis was conducted by the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling (TUNEL) method using the BD ApoAlert DNA Fragmentation Assay Kit. The stained cells were analyzed by flow cytometer (Becton Dickinson and Co.) and fluorescence microscope (Nikon Eclipse TE 300, Germany) equipped with a filter system (emission filter 520 nm) at 20x magnification.

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Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma using ApoAlert™ DNA Fragmentation Assay Kit from Takara Bio Inc.

Paper title
Subamolide A Induces Mitotic Catastrophe Accompanied by Apoptosis in Human Lung Cancer Cells
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Manufacturer protocol

Download the product protocol from Takara Bio Inc for ApoAlert™ DNA Fragmentation Assay Kit below.

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Videos

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