in situ Cell Death Detection Kit, POD

TUNEL assay cell type - PANC-1 human pancriatic cancer

Experiment
TUNEL assay cell type - PANC-1 human pancriatic cancer
Product
in situ Cell Death Detection Kit, POD from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Protocol tips
Incubate tissue section for 30 min at +37°C with Proteinase K.

Add Tunel reaction and incubate for 60 min at +37°C in a humidified atmosphere in the
dark
Downstream tips
To analyze use an excitation wavelength in the range of 450–500 nm and detection in the range of 515 – 565 nm (green).

Publication protocol

The TUNEL assay was performed using the In Situ Cell Death Detection kit, POD (Roche-Applied-Science, Mannheim, Germany), according to the manufacturer's instructions. The paraffin embedding spheres were deparaffinized and rehydrated; then tissues sections were incubated with proteinase K solution (10–20 μg/mL) for 30 min. Tissues were then rinsed twice in PBS and reacted with 50 μL of the TUNEL reaction mixture at room temperature for 60 min in a dark, humidified chamber. Sections were again rinsed in PBS and incubated for 30 min with 50 μL of the Converter-POD (Roche-Applied-Science) and followed by 3-amino-9-ethylcarbazole (AEC). Sections were then counterstained with hematoxylin. As negative controls, corresponding sections were treated in the same way without terminal deoxynucleotidyl transferase.

Full paper   Login or join for free to view the full paper.

Reviews

in situ Cell Death Detection Kit, POD from Sigma-Aldrich has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Ercole Udinesi Italy

Are 10 week old samples in formalin still usable?

I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - PANC-1 human pancriatic cancer using in situ Cell Death Detection Kit, POD from Sigma-Aldrich.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for in situ Cell Death Detection Kit, POD below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing TUNEL assay cell type - PANC-1 human pancriatic cancer using in situ Cell Death Detection Kit, POD from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms