QIAamp DNA Blood BioRobot MDx Kit (12)

DNA isolation / purification Tissue - blood / plasma

Experiment
DNA isolation / purification Tissue - blood / plasma
Product
QIAamp DNA Blood BioRobot MDx Kit (12) from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
Follow manufacturer's instructions

Publication protocol

Illumina infinium human methylation 450K beadchip: Blood samples were obtained from each participant via venipuncture and sent to Roswell Park Cancer Institute for DNA extraction. Genomic DNA was isolated from 0.3 ml of whole blood using the Qiagen BioRobot Universal System and the QIAamp DNA blood BioRobot MDx Kit (Qiagen, Valencia, CA, USA) following the manufacturer’s recommended protocol. DNA methylation profiling was performed by Roswell Park Cancer Institute using the Human Methylation 450 K BeadChip (Illumina, San Diego, CA, USA). DNA extraction and methylation profiling were done blinded to group assignment. Five hundred nanogram of high-quality genomic DNA measured by picogreen quantitation (Life technologies, Grand Island, NY, USA) was bisulphite converted, amplified, fragmented and hybridized to the Illumina Infinium Human Methylation 450 K Beadchip using standard Illumina protocol. Data were processed using Illumina's GenomeStudio methylation module (v1.9.0).

Full paper   Login or join for free to view the full paper.

Reviews

QIAamp DNA Blood BioRobot MDx Kit (12) from Qiagen has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Denmark

What DNA isolation kit would work for insect samples?

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Tissue - blood / plasma using QIAamp DNA Blood BioRobot MDx Kit (12) from Qiagen.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Blood BioRobot MDx Kit (12) below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA isolation / purification Tissue - blood / plasma using QIAamp DNA Blood BioRobot MDx Kit (12) from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms