miRNeasy Serum/Plasma Advanced Kit (50)

RNA isolation / purification Tissue - Livestock Blood / Serum / Plasma / Buffy coat

Experiment
RNA isolation / purification Tissue - Livestock Blood / Serum / Plasma / Buffy coat
Product
miRNeasy Serum/Plasma Advanced Kit (50) from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
Follow manufacturer’s instructions

Publication protocol

RNA extraction: RNA was extracted from either fresh or frozen plasma according to the manufacturer’s instructions using 6 commercially available kits; Kit A: miRNeasy Serum/Plasma (Qiagen, Germany), Kit B: miRNeasy Serum/Plasma Advanced (Qiagen, Germany), Kit C: Quick-cfRNA Serum & Plasma (Zymo Research, USA), Kit D: Isolate II miRNA (Bioline, Australia), Kit E: PureLink RNA Mini kit (Invitrogen, USA), Kit F: Monarch Total RNA Miniprep kit (New England BioLabs, USA). All extractions were performed in duplicate for each animal, except for the PureLink and Monarch kits due to sample and reagent availability. Following extraction and elution of RNA, samples were immediately frozen at −80 °C.
Kit A: miRNeasy Serum/Plasma kit
Briefly, Total RNA was isolated from 200 µl of plasma as per the manufacturer’s recommendations and eluted in 14 µl of RNase-free water.
Kit B: miRNeasy Serum/Plasma Advanced kit
The miRNeasy Serum/Plasma Advanced kit is a phenol-free alternative to the original miRNeasy Serum/Plasma kit. Total RNA was extracted from 200 µl of plasma according to the manufacturer’s protocol and eluted in 20 µl of RNase-free water.

Full paper   Login or join for free to view the full paper.

Reviews

miRNeasy Serum/Plasma Advanced Kit (50) from Qiagen has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Livestock Blood / Serum / Plasma / Buffy coat using miRNeasy Serum/Plasma Advanced Kit (50) from Qiagen.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Qiagen for miRNeasy Serum/Plasma Advanced Kit (50) below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Tissue - Livestock Blood / Serum / Plasma / Buffy coat using miRNeasy Serum/Plasma Advanced Kit (50) from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms