RNeasy DSP FFPE Kit

RNA isolation / purification Tissue - Human FFPE tissue

Experiment
RNA isolation / purification Tissue - Human FFPE tissue
Product
RNeasy DSP FFPE Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Publication protocol

RNA extraction and sample handling: RNA extraction was performed using the RNeasy DSP FFPE kit on 4 to 5 μm thick FFPE sections with a tumor area between 100 and 500 mm2. Macrodissection was performed if necessary to attain a minimum of 80% tumor area. All steps outlined in the RNeasy DSP FFPE kit protocol were performed at room temperature (15 to 25 °C). Once extracted the RNA samples could either be tested immediately with the RT‐PCR kit or stored at −90 to −65 °C and a maximum of five freeze–thaw cycles were permissible.

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy DSP FFPE Kit below.

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Videos

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