About nuclease-free water in DNA quantification

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5 years ago

Dorota Ostrowska

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About nuclease-free water in DNA quantification

Hi everyone! I have a question about DNA quantification. Do I need to use nuclease-free water for DNA quantification and are there any repercussions if I don’t use any?

Experiment: DNA quantification Human - SH-SY5Y

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**Dr. Jasmine Tomar** · Answer 1 · 2019-05-25T13:39:49+00:00

The short answer is yes. If you intend to do a precise quantification you should use nuclease-free water in order to avoid having your DNA slightly degrade, which in turn will alter the DNA concentration. However, if you are using it for routine techniques and you measure the DNA reasonably quickly, using nuclease-free water should not matter that much.

**Dorota Ostrowska** · Answer 2 · 2019-06-12T18:52:12+00:00

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Thank you for your help Dr. Tomar. I will keep that in mind!

Answered 5 years ago

Dorota Ostrowska

**Dr. Jasmine Tomar** · Answer 3 · 2019-05-25T13:39:49+00:00

The short answer is yes. If you intend to do a precise quantification you should use nuclease-free water in order to avoid having your DNA slightly degrade, which in turn will alter the DNA concentration. However, if you are using it for routine techniques and you measure the DNA reasonably quickly, using nuclease-free water should not matter that much.