Discussion
5 years ago
Anders Bergman
3I am planning on doing an analysis via crystallization on a trimeric protein complex from mammalian tissue culture cells. Is it possible to express and purify such a protein from mammalian tissue?
Experiment: Protein expression and purification Mammalian cells - HEK 293 Mt-PA
Top comment
You may able to express but several factors may effect the expression like the size of the complex, Glycosylation sites, codon usage, etc, I would rather suggest split the complex to monomeric/single units or domains and self assemble after expression.
Answered 5 years ago
Ganesh Ram Visweswaran
3 Comments
Thank you for your answer! I will split the complex into monomeric domains as you suggested
Answered 5 years ago
Anders Bergman
You may able to express but several factors may effect the expression like the size of the complex, Glycosylation sites, codon usage, etc, I would rather suggest split the complex to monomeric/single units or domains and self assemble after expression.
Answered 5 years ago
Ganesh Ram Visweswaran
You may able to express but several factors may effect the expression like the size of the complex, Glycosylation sites, codon usage, etc, I would rather suggest split the complex to monomeric/single units or domains and self assemble after expression.
Answered 5 years ago
Ganesh Ram Visweswaran
Can you help?