About expressing a trimeric protein complex from mammalian tissue

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5 years ago

Anders Bergman

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About expressing a trimeric protein complex from mammalian tissue

I am planning on doing an analysis via crystallization on a trimeric protein complex from mammalian tissue culture cells. Is it possible to express and purify such a protein from mammalian tissue?

Experiment: Protein expression and purification Mammalian cells - HEK 293 EGFR

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**Ganesh Ram Visweswaran** · Answer 1 · 2019-06-14T15:28:27+00:00

You may able to express but several factors may effect the expression like the size of the complex, Glycosylation sites, codon usage, etc, I would rather suggest split the complex to monomeric/single units or domains and self assemble after expression.

**Anders Bergman** · Answer 2 · 2019-06-20T10:59:02+00:00

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Thank you for your answer! I will split the complex into monomeric domains as you suggested

Answered 5 years ago

Anders Bergman

**Ganesh Ram Visweswaran** · Answer 3 · 2019-06-14T15:47:31+00:00

You may able to express but several factors may effect the expression like the size of the complex, Glycosylation sites, codon usage, etc, I would rather suggest split the complex to monomeric/single units or domains and self assemble after expression.

**Ganesh Ram Visweswaran** · Answer 4 · 2019-06-14T15:28:27+00:00

You may able to express but several factors may effect the expression like the size of the complex, Glycosylation sites, codon usage, etc, I would rather suggest split the complex to monomeric/single units or domains and self assemble after expression.