How can I preserve leukocytes for DNA methylation profiling?

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5 years ago

Jody Hancock

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How can I preserve leukocytes for DNA methylation profiling?

I would like to preserve leukocytes for future epigenetic analysis. How can I preserve them effectively in order to perform DNA methylation profiling at a later time?

Experiment: DNA methylation profiling Gene specific profiling - Hep3B SPRY1

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**Kriztian Huisman** · Answer 1 · 2019-06-27T06:18:02+00:00

For DNA methylation profiling you only need the DNA, so a good way is to isolate the DNA first and then store the DNA at -20°C. You can also snap-freeze the cells using liquid nitrogen and then store the cells at -20°C and isolate the DNA later. For single cell DNA methylation profiling, probably fresh cells are the best,

**Irvin S. Herold** · Answer 2 · 2020-04-14T13:04:30+00:00

If you are going to perform DNA methylation profiling, i would advice you to promptly extract DNA in order to avoid exposition to any extra stress conditions that could potentially induce unwanted methylation changes in your samples. DNA can be stored safely for long times in the freezer.

**Kriztian Huisman** · Answer 3 · 2019-06-27T06:18:02+00:00

For DNA methylation profiling you only need the DNA, so a good way is to isolate the DNA first and then store the DNA at -20°C. You can also snap-freeze the cells using liquid nitrogen and then store the cells at -20°C and isolate the DNA later. For single cell DNA methylation profiling, probably fresh cells are the best,