When should I be harvesting the tissue before using it for the most optimal results?

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5 years ago

Jesualdo Hernández

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When should I be harvesting the tissue before using it for the most optimal results?

I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?

Experiment: Protein isolation Tissue - Human aortic endothelial cells

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**Hari Krishna** · Answer 1 · 2019-07-05T18:59:34+00:00

HIF proteins at the presence of atmospheric oxygen concentration have a half-life of about five minutes. This means that you should harvest your tissue samples within a couple of minutes otherwise the proteins will start degrading.