1 year ago
Whether or not knockdowns from shRNA gene silencing are permanent depends on the exact vector used. Delivery by (non integrase-deficient) lentivirus should be permanent on a theoretical basis, as the shDNA integrates into the cell's genome. However, continued transduction or other frequent treatments that edit or mutate the cell's DNA might have varying effects on the knockdown. While the probability should be low you could consider confirming the status of your knockdown in intervals of weeks to months, if you continue such experiments.