Discussion
5 years ago
Israel Lev
2The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?
Experiment: DNA isolation / purification Bacteria - Gram negative Klebsiella
Top comment
Hi Israel, Interesting question indeed!
Answered 5 years ago
Dhr. L.S. Petitiaux
2 Comments
Start with more cells or run multiple purifications and combine the results. If this is plasmid DNA, be sure to choose a high copy form. Remember to rely heavily on cold precipitation temps as well. Follow the time events in your recipe accurately.
Answered 4 years ago
Hayo Weekers
Hi Israel, Interesting question indeed!
Answered 5 years ago
Dhr. L.S. Petitiaux
Can you help?