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5 years ago

2

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Experiment: DNA isolation / purification Bacteria - Gram positive Bacillus subtilis

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Top comment

1

Hi Israel, Interesting question indeed!

Answered 5 years ago

Dhr. L.S. Petitiaux

2 Comments

1

Start with more cells or run multiple purifications and combine the results. If this is plasmid DNA, be sure to choose a high copy form. Remember to rely heavily on cold precipitation temps as well. Follow the time events in your recipe accurately.

Answered 4 years ago

1

Hi Israel, Interesting question indeed!

Answered 5 years ago

Dhr. L.S. Petitiaux

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