How to avoid genomic DNA contamination when isolating mitochondrial DNA?

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5 years ago

Jean Rossignol

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How to avoid genomic DNA contamination when isolating mitochondrial DNA?

Greetings! I am trying to isolate mitochondrial DNA from S. cerevisiae while avoiding contamination from genomic DNA. Any and all help is greatly appreciated.

Experiment: DNA isolation / purification Yeast - Pichia pastoris

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score 0 · Answer 1 · 2019-08-14T11:07:51+00:00

I would suggest that you run a real-time PCR assay with mtDNA and nuclear DNA primer sets. By doing that you will enrich the mtDNA/nDNA and you will be able to verify the purity of your preparation. Then you can run the extracted DNA on an agarose gel and extract only the mtDNA band(s).