That is not possible since multiplex PCR requires different sets of primers and different fluorophores on each matching probe of the sets. Also, you will need to simultaneously detect your different targets via a multichannel qPCR detection system. Using only SYBR green you will only detect it on one specific channel.
No, it’s not really possible, as multiplex depends on different fluorescent dyes to differentiate between the targets, and Sybr Green fluoresces at just one wavelength, so your instrument will not be able to differentiate between your different targets.
That is not possible since multiplex PCR requires different sets of primers and different fluorophores on each matching probe of the sets. Also, you will need to simultaneously detect your different targets via a multichannel qPCR detection system. Using only SYBR green you will only detect it on one specific channel.
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That is not possible since multiplex PCR requires different sets of primers and different fluorophores on each matching probe of the sets. Also, you will need to simultaneously detect your different targets via a multichannel qPCR detection system. Using only SYBR green you will only detect it on one specific channel.
Answered 5 years ago
Lauren Dobson
2 Comments
No, it’s not really possible, as multiplex depends on different fluorescent dyes to differentiate between the targets, and Sybr Green fluoresces at just one wavelength, so your instrument will not be able to differentiate between your different targets.
Answered 4 years ago
Eleni Papadopoulou
That is not possible since multiplex PCR requires different sets of primers and different fluorophores on each matching probe of the sets. Also, you will need to simultaneously detect your different targets via a multichannel qPCR detection system. Using only SYBR green you will only detect it on one specific channel.
Answered 5 years ago
Lauren Dobson
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