Reactivate dormant Lactobacillus Paracasei

Hello Janardhan, You should be able to do that by inoculating a colony in MRS broth and then incubating it for a week at 37 °C. Check the tube every day and if you see some turbidity subculture it 3-4 times in order to get an active culture.

Try this: Incubate MRS broth anaerobically at least overnight. Inoculate with as large an inoculum of L. paracasei as you can spare. Incubate anaerobically at between 30 to 37 °C until you observe inreased turbidity. Passage similarly two or three more times. Then transfer to agar plates.

Hello Janardhan, You should be able to do that by inoculating a colony in MRS broth and then incubating it for a week at 37 °C. Check the tube every day and if you see some turbidity subculture it 3-4 times in order to get an active culture.