Will presence of EDTA effect cDNA synthesis

There is a chance that EDTA might interfere with cDNA synthesis. In order to avoid that you could increase the volume of the cDNA reaction to 50 μl and add extra Magnesium. Make sure that you do not heat the sample in the presence of Magnesium though and only do it before adding it. If this does not work you should use the designated elution buffer of your kit after extracting the RNA instead of TE buffer. Alternatively, you can also use RNAse free water. Hope this helps.

Hi, the EDTA acts as a metal chelator and will inhibit the activity of most enzymes, including reverse transcriptase. I would do a bead clean up and elute your RNA 10 mM TRIS HCL and 50 mM Nacl solution, or something to that end with a neutral pH. You can use the agencourt RNA XP beads for the bead cleanup by beckman coulter.

There is a chance that EDTA might interfere with cDNA synthesis. In order to avoid that you could increase the volume of the cDNA reaction to 50 μl and add extra Magnesium. Make sure that you do not heat the sample in the presence of Magnesium though and only do it before adding it. If this does not work you should use the designated elution buffer of your kit after extracting the RNA instead of TE buffer. Alternatively, you can also use RNAse free water. Hope this helps.