Discussion

5 years ago

1

DNA isolation column clogged

During centrifugation, the column got clogged and I was unable to continue with the protocol. How can I unclog it?

Experiment: DNA isolation / purification Cells - Immortalized cell lines Renal cortical tubule epithelial cells

Comment

1 Comment

1

Try not to exceed the recommended volume of starting material that is listed by the manufacturer and if this still occurs try to use an even smaller volume. You can try to fix the clogged one by increasing the g force and centrifuging the sample for a longer period of time. Also, make sure that the columns actually work. There is always the chance that you got a faulty batch and you should contact the manufacturer about it.

Answered 5 years ago

Can you help?

Start your discussion

Share your thoughts or question with experts in your field

Start discussion
Become shareholder Discussions About us Contact Privacy Terms