Multiple gene silencing using ShRNA

Question

Discussion

5 years ago

A.C.Burton

1

Multiple gene silencing using ShRNA

Hello, can someone here help me? I am trying to silence e-selectin and ICAM-1 in endothelial cells. I would like to know if this is possible using shRNA

Experiment: shRNA gene silencing Mouse - RGC-5 Syn G (Exon 3)

Comment

1 Comment

Can you help?

Start your discussion

Share your thoughts or question with experts in your field

Start discussion

**S Janardhan** · Answer 1 · 2019-12-15T20:32:16+00:00

Hello Burton, Generally it should be possible to target multiple genes however all of your constructs will contain the same selection marker. As a result, it would be impossible to determine which cells within your culture got both of the virus types.