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5 years ago

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Multiple gene silencing using ShRNA

Hello, can someone here help me? I am trying to silence e-selectin and ICAM-1 in endothelial cells. I would like to know if this is possible using shRNA

Experiment: shRNA gene silencing Mouse - Prostate cancer cell lines (DU145 and PC3) CD24 lentiviral particles

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Hello Burton, Generally it should be possible to target multiple genes however all of your constructs will contain the same selection marker. As a result, it would be impossible to determine which cells within your culture got both of the virus types.

Answered 4 years ago

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