RNA isolation from tissue

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5 years ago

Paul G. Macon

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RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Experiment: RNA isolation / purification Tissue - Rat Brainstem

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**T. Enestam** · Answer 1 · 2020-01-26T13:53:58+00:00

No, you should not try to homogenize the tissue at room temperature. Instead, you should try homogenize the tissue in ice. As for using liquid nitrogen, you can use dry ice instead of nitrogen.

**Yuri van der Kloet** · Answer 2 · 2020-05-04T09:37:59+00:00

Yeah, that is usually sufficient although i do not recall the details of trizol extraction. There is a precipitation step on the soluble phase and then you spin down the pellet, air dry it and don’t overdry it after 70% etoh wash. Bring it up in a small volume of TE rnase free, or some other suitable storage buffer. Keep it at -80. As long as you don’t freeze thaw alot, it will keep for a long time

**T. Enestam** · Answer 3 · 2020-01-26T13:53:58+00:00

No, you should not try to homogenize the tissue at room temperature. Instead, you should try homogenize the tissue in ice. As for using liquid nitrogen, you can use dry ice instead of nitrogen.