PCR Hot start PCR

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality hot-start DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction

Get tips on using Hot Start Taq DNA Polymerase to perform PCR Hot start PCR - Mammalian DNA

Get tips on using TEMPase Hot Start Master Mix BLUE to perform PCR Hot start PCR - Bacterial DNA

Get tips on using Phusion Hot Start II DNA Polymerase to perform PCR Hot start PCR - Bacterial DNA

Get tips on using Q5® Hot Start High-Fidelity 2X Master Mix to perform PCR Hot start PCR - Mammalian DNA

Get tips on using Hot StarTaq Master Mix Kit (2500 U) to perform PCR Hot start PCR - Mammalian DNA

Get tips on using KAPA LongRange to perform PCR Hot start PCR - Mammalian DNA

Get tips on using HotStarTaq DNA Polymerase (25000) to perform PCR Hot start PCR - Bacterial DNA

Get tips on using HotStarTaq Plus DNA Polymerase to perform PCR Hot start PCR - Bacterial DNA