Get tips on using Anti-Human CD56 (NCAM) APC-eFluor® 780 to perform Flowcytometry CD56 (NCAM) - Mouse / IgG1, kappa Human APC-eFluor 780
Get tips on using Purified Mouse Anti-Human CD56 to perform Flow cytometry Anti-bodies Human - CD56
Get tips on using V450 Mouse Anti-Human CD56 to perform Flow cytometry Anti-bodies Human - CD56
Get tips on using PE-Cy™7 Mouse Anti-Human CD56 to perform Flow cytometry Anti-bodies Human - CD56
Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).
Get tips on using Alexa Fluor 700-labeled anti-CD16 to perform Flowcytometry CD16 - Mouse /IgG1, kappa Human Alexa Fluor 700
Get tips on using Anti-Human CD282 (TLR2) FITC to perform Flowcytometry TLR2 (CD282) - Mouse / IgG1, kappa Human FITC
Get tips on using Anti-Human CD3 PE-Cyanine7 to perform Flowcytometry CD3 - Mouse / IgG1, kappa Human PE-Cyanine7
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