Immunohistochemistry Estrogen receptor (ER) Rabbit Human

Get tips on using Anti-Estrogen Receptor (ER) (SP1), Rabbit Monoclonal Primary Antibody to perform Immunohistochemistry Estrogen receptor (ER) - Rabbit Human -NA-

Get tips on using CONFIRM anti-Estrogen Receptor (ER) (SP1) Rabbit Monoclonal Primary Antibody to perform Immunohistochemistry Human - ER

Get tips on using Estrogen Receptor to perform Immunohistochemistry Human - ER

Get tips on using Estrogen Receptor α (D8H8) Rabbit mAb #8644 to perform ChIP Anti-bodies ERα

Get tips on using Estrogen Receptor alpha Monoclonal Antibody (SP1) to perform Immunohistochemistry Human - ER

Get tips on using Anti-Estrogen Receptor α Antibody to perform Immunohistochemistry Mouse - ERα

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Estrogen Receptor alpha Antibody (F-10): sc-8002 to perform Immunohistochemistry Mouse - ERα

Get tips on using Estrogen Receptor alpha Monoclonal Antibody (SP1) to perform Western blotting Estrogen Receptor