Immunohistochemistry Vimentin (3B4) Mouse Human

Get tips on using Anti-vimentin to perform Immunohistochemistry Vimentin (3B4) - Mouse Human -NA-

Get tips on using Vimentin Antibody to perform Immunohistochemistry Vimentin - Mouse Human -NA-

Get tips on using Vimentin (D21H3) XP® Rabbit mAb to perform Immunohistochemistry Vimentin - Rabbit Human / mouse -NA-

Get tips on using Anti-human vimentin (clone V9) to perform Flow cytometry Anti-bodies Human - Vimentin

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).

Get tips on using Anti-Vimentin antibody (ab137321) to perform Flow cytometry Anti-bodies Human - Vimentin

Get tips on using Vimentin Monoclonal Antibody (V9) to perform Western blotting Vimentin

Get tips on using Vimentin Monoclonal Antibody (V9), Biotin to perform Western blotting Vimentin

Get tips on using Vimentin Antibody (5G3F10): sc-66002 to perform Western blotting Vimentin

Get tips on using Vimentin Antibody (E-5): sc-373717 to perform Western blotting Vimentin