FACS

Get tips on using CD14 Antibody PE-labelled to perform FACS CD14 - Mouse Human PE

Get tips on using Phalloidin–Tetramethylrhodamine B isothiocyanate to perform FACS Phalloidin - Amanita phalloides Human TRITC

Get tips on using PerCP-Cy5-labeled CD66b to perform FACS CD66b - Mouse Human PerCP-Cy5

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using Thyroid Transcription Factor-1 to perform Immunohistochemistry Human - TTF-1

Get tips on using ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA to perform ELISA Human - GDNF

Get tips on using Fibroblast Growth Factor 21 Mouse/Rat ELISA to perform ELISA Rat - FGF-21

Get tips on using Human VWF / von Willebrand Factor ELISA Kit to perform ELISA Human - VWF-A2

Get tips on using Mouse Epidermal Growth Factor Receptor (EGFR) ELISA Kit to perform ELISA Mouse - EGFR

Get tips on using TransAM® ER Transcription Factor ELISA Kits to perform ELISA Human - Estrogen receptor (ESRs)