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Flowcytometry CD16 Mouse /IgG1, kappa

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Get tips on using Alexa Fluor 700-labeled anti-CD16 to perform Flowcytometry CD16 - Mouse /IgG1, kappa Human Alexa Fluor 700

Products BioLegend Alexa Fluor 700-labeled anti-CD16

Get tips on using Anti-Human CD282 (TLR2) FITC to perform Flowcytometry TLR2 (CD282) - Mouse / IgG1, kappa Human FITC

Products eBioscience Anti-Human CD282 (TLR2) FITC

Get tips on using Anti-Human CD3 PE-Cyanine7 to perform Flowcytometry CD3 - Mouse / IgG1, kappa Human PE-Cyanine7

Products eBioscience Anti-Human CD3 PE-Cyanine7

Get tips on using Anti-Human CD284 (TLR4) to perform Flowcytometry TLR4 (CD284) - Mouse / IgG1, kappa Human Brilliant violet 421

Products eBioscience Anti-Human CD284 (TLR4)

Get tips on using Anti-Human CD56 (NCAM) APC-eFluor® 780 to perform Flowcytometry CD56 (NCAM) - Mouse / IgG1, kappa Human APC-eFluor 780

Products eBioscience Anti-Human CD56 (NCAM) APC-eFluor® 780

Get tips on using InVivoMAb anti-mouse CD16/CD32 to perform Flow cytometry Anti-bodies Mouse - CD16/CD32

Products Bio X Cell InVivoMAb anti-mouse CD16/CD32

Get tips on using Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) to perform Flow cytometry Anti-bodies Mouse - CD16/CD32

Products BD Biosciences Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™)

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).

Proteins Flow cytometry Anti-bodies Mouse CD16/CD32

Cellular assays Cell Isolation CD16+ Monocyte

Cellular assays Cell Isolation CD56+CD16+ NK Cell

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