PCR Preamplification of cDNA

Get tips on using QuantiFast Pathogen PCR +IC Kit (400) to perform PCR Quantitative real-time PCR - Bacterial DNA

Get tips on using miScript SYBR Green PCR Kit (2000) to perform PCR Quantitative real-time PCR - Mammalian DNA

Get tips on using SYBR™ Green PCR Master Mix to perform PCR Quantitative real-time PCR - Bacterial DNA

Get tips on using SYBR™ Green PCR Master Mix to perform PCR Quantitative real-time PCR - Mammalian DNA

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

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Get tips on using QuantiNova SYBR Green RT-PCR Kit (2500) to perform PCR Quantitative real-time PCR - Viral