rna-isolation-purification-tissue-mouse-mammary-glands

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Tissue - Human Blood / Serum / Plasma / Buffy coat

Get tips on using Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE to perform RNA isolation / purification Tissue - Rat Kidney

Get tips on using AllPrep DNA/RNA/Protein Mini Kit (50) to perform DNA isolation / purification Tissue - brain

Get tips on using QIAamp Fast DNA Tissue Kit to perform DNA isolation / purification Tissue - colon

Get tips on using QIAamp DNA FFPE Tissue Kit to perform DNA isolation / purification Tissue - heart

Get tips on using QIAamp DNA FFPE Tissue Kit to perform DNA isolation / purification Tissue - bone

Get tips on using QIAamp DNA FFPE Tissue Kit to perform DNA isolation / purification Tissue - lung

Get tips on using QIAamp DNA FFPE Tissue Kit to perform DNA isolation / purification Tissue - liver

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.