DNA transfection Mammalian cells

Get tips on using StepUp 50bp DNA Ladder to perform DNA Ladder 50 bp

Get tips on using BenchTop 100bp DNA Ladder to perform DNA Ladder 100 bp

Get tips on using 1kb DNA Step Ladder to perform DNA Ladder 1 kb

Get tips on using 100bp DNA Step Ladder to perform DNA Ladder 100 bp

Get tips on using 100 bp DNA Ladder to perform DNA Ladder 100 bp

Get tips on using 123 bp DNA Ladder to perform DNA Ladder 123 bp

Get tips on using 200bp DNA Step Ladder to perform DNA Ladder 200 bp

Get tips on using BenchTop 1kb DNA Ladder to perform DNA Ladder 1 kb

Get tips on using 1 kb DNA Ladder to perform DNA Ladder 1 kb

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. Multiplexing such a reaction amplifies the design challenges where one target requires 3 primers, which should be exclusively bound nowhere in the template DNA or to each other. Similarly, two targets require 6, three require 9, and so on. Each amplicon needs to be either a different size (for gels) or labeled with a different fluorescent tag that is spectrally distinct from the others in the reaction. Further complicating this, different targets in the reaction can compete with each other for resources and causes more challenges in the detection of amplicons. However, with proper primer designing, their validation, optimize quality and concentration of the enzyme and buffers certainly lead to a successful multiplex PCR reaction.