Flowcytometry CD14 Mouse / IgG2a Human

Get tips on using CD49d Antibody, anti-human, Biotin to perform Flow cytometry Anti-bodies Human - CD49d

Get tips on using PE anti-human CD49d Antibody to perform Flow cytometry Anti-bodies Human - CD49d

Get tips on using FITC anti-human CD4 Antibody to perform Flow cytometry Anti-bodies Human - CD4

Get tips on using EasySep™ Release Human CD19 Positive Selection Kit to perform Cell Isolation B cell

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using FITC Rat Anti-Human CD49f to perform Flow cytometry Anti-bodies Human - CD49f/ITGA6

Get tips on using Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse to perform Western blotting ERK

Get tips on using PerCP-Cy5-labeled CD66b to perform FACS CD66b - Mouse Human PerCP-Cy5

Get tips on using EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion to perform Cell Isolation Monocyte

Get tips on using Human ICAM1 ELISA Kit (CD54) (ab100640) to perform ELISA Human - ICAM-1/CD54