Generally it has been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Use of enzymes to disrupt cell wall can alter gene expression profiles. Therefore, physical disruption can result in high quality RNA for all downstream processing. Use of DNAse and proteinase K will remove traces of DNA contamination and proteins respectively.
Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Tissue - mouse liver
Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Tissue - mouse kidney
Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Tissue - mouse brain
Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Tissue - mouse thymus
Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Tissue - mouse aorta
Get tips on using SuperSignal™ Enhanced Molecular Weight Protein Ladder to perform Protein Ladder Unstained
Get tips on using WesternSure® Pre-stained Chemiluminescent Protein Ladder to perform Protein Ladder Immunofluorescence
Get tips on using Chameleon® Vue Pre-stained Protein Ladder to perform Protein Ladder Immunofluorescence
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