Get tips on using ON-TARGETplus Mouse Zbp1 (58203) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - 3T3-SA Zbp1/Dai
Get tips on using BUV395 Mouse Anti-Human CD123 to perform Flow cytometry Anti-bodies Human - CD123/IL3-R
Get tips on using PE Mouse Anti-Human CD31 to perform Flow cytometry Anti-bodies Human - CD31/PECAM-1
Get tips on using Mouse Gene Expression v2 4x44K Microarray Kit to perform Microarray Gene expression arrays - Mouse liver tissue Cyanine-3-CTP
Get tips on using Monoclonal Mouse Anti-Human Hepatocyte (Concentrate) Clone OCH1E5 to perform Immunohistochemistry Mouse - Hepatocyte
Get tips on using Mouse MCP-1 PicoKine™ Fast ELISA Kit to perform ELISA Mouse - MCP1
Get tips on using Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit to perform ELISA Mouse - MCP1
Get tips on using Mouse Epidermal Growth Factor Receptor (EGFR) ELISA Kit to perform ELISA Mouse - EGFR
Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
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