siRNA / miRNA gene silencing Human HT-1080

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Get tips on using ON-TARGETplus Mouse Bnip3 (12176) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - RAW264.7 BNIP3

Products Horizon Discovery Ltd. ON-TARGETplus Mouse Bnip3 (12176) siRNA - SMARTpool

Get tips on using ON-TARGETplus Mouse Rheb (19744) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - RAW264.7 Rheb

Products Horizon Discovery Ltd. ON-TARGETplus Mouse Rheb (19744) siRNA - SMARTpool

Get tips on using HIF-1α siRNA (r) to perform siRNA / miRNA gene silencing Rat - Brain endothelial cells HIF-1α Lipid

Products Santa Cruz Biotechnology HIF-1α siRNA (r)

Short hairpin or small hairpin RNA (shRNA) is artificial RNA, which has a hairpin loop structure, and uses inherent microRNA (miRNA) machinery to silence target gene expression. This is called RNA interference (RNAi). These can be delivered via plasmids or viral/bacterial vectors. Challenges in shRNA-mediated gene silencing include 1. Off-target silencing, 2. Packaging shRNA encoding lentivirus, and 3. Stable transduction in cells. RNAi has been designed to have anywhere from 19-27 bs, but the most effective design has 19 bp. In case commercial shRNAs are not available, potential target sites can be chosen within exon, 5’- or 3’ UTR, depending on which splice variants of the gene are desired. One should use the latest algorithms and choose at least two different sequences, targeting different regions, in order to have confidence in overcoming off-target effects. A BLAST search after selecting potential design will eliminate potential off-target sequences. For the second challenge, sequencing the vector using primers for either strand (50-100 bp upstream) is suggested, along with using enzymatic digestion on agarose gel for the vector. Next, once the shRNA-containing vector is packaged in a virus, it is important to check the viral titer before transduction. Finally, using a marker in the lentiviral vector (fluorescent protein or antibiotic resistance), along with qPCR for target gene expression can help in determining the efficacy of transduction and shRNA on its target site.

RNA shRNA gene silencing Mouse FL83B HtrA2

Get tips on using PureHelix™ RNA Extraction Solution to perform RNA isolation / purification Cells - immortalized HT-1080

Products NanoHelix PureHelix™ RNA Extraction Solution

Get tips on using 48-Well Micro Chemotaxis Chamber to perform Cell migration / Invasion cell type - HT-1080

Products Neuro Probe 48-Well Micro Chemotaxis Chamber

Get tips on using Silencer® Select- Gdf10 siRNA to perform siRNA / miRNA gene silencing Mouse - 3T3-L1 BMP-3b/GDF10

Products Thermo Fisher Scientific Silencer® Select- Gdf10 siRNA

Get tips on using C/EBP β siRNA (r) to perform siRNA / miRNA gene silencing Rat - Glial cells C/EBP‐β

Products Santa Cruz Biotechnology C/EBP β siRNA (r)

Get tips on using FlexiTube GeneSolution GS26354 for GNL3 to perform siRNA / miRNA gene silencing Human - MDA-MB-231 GNL3

Products Qiagen FlexiTube GeneSolution GS26354 for GNL3

Get tips on using siGENOME Rat Acvr1c (245921) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - INS-1 Alk7/Acvr1c

Products Horizon Discovery Ltd. siGENOME Rat Acvr1c (245921) siRNA - SMARTpool

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