siRNA / miRNA gene silencing Human PC-3

RNAi or RNA interference is a common method to suppress gene expression in vitro/in vivo by utilizing the inherent microRNA machinery, without introducing a total gene knockout. miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid-mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time-consuming, but provide a more permanent expression of RNAi in the cells and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines.

Get tips on using ON-TARGETplus Rat Dlc1 (58834) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 Dlc1

Get tips on using ON-TARGETplus Rat Rhoa (117273) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 Rhoa

Get tips on using ON-TARGETplus Rat Rhoc (295342) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 RhoC

Get tips on using ON-TARGETplus Rat Pak1 (29431) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 Pak1

Get tips on using ON-TARGETplus Rat Nos3 (24600) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - NPC Nos3

Get tips on using ON-TARGETplus Rat Klf15 (85497) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - NRCM Klf15

Get tips on using ON-TARGETplus Mouse Stat5b (20851) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - FL83B Stat5b

Get tips on using ON-TARGETplus Mouse Cdh5 (12562) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - MS1 Cdh5

Get tips on using ON-TARGETplus Mouse Cd84 (12523) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - M210B4 Cd84