siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using Gabpb1 siRNA to perform siRNA / miRNA gene silencing Rat - Schwann cells Nrf2

Get tips on using Ccr2 siRNA to perform siRNA / miRNA gene silencing Rat - Glial cells CCR2

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using Lipofectamine® RNAiMAX Transfection Reagent to perform siRNA / miRNA gene silencing Human - siRNA negative control Lipid

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Cells - primary human umbilical vein endothelial cells

Get tips on using Quick-RNA Microprep Kit to perform RNA isolation / purification Cells - primary human pulmonary artery endothelial cells

Get tips on using RNeasy Plus Micro Kit to perform RNA isolation / purification Cells - primary human lung microvascular endothelial cells

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Cells - primary human dermal microvascular endothelial cells