Protein expression and purification Insect cells Hi5

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Get tips on using pET-INP-cellulase to perform Protein Expression Prokaryotic cells - E. coli INP-cellulase

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using pLEXSY-hyg-2-hAm to perform Protein Expression Eukaryotic cells - Iranian lizard Leishmania cells recombinant human amelogenin

Get tips on using pMHL_2002-bfloGFPa1 to perform Protein Expression Eukaryotic cells - T. pseudonana IbpA DR2 antigen from Histophilus somni

Get tips on using pMJS205 to perform Protein Expression Prokaryotic cells - E. coli S. cerevisiae SOX Erv1p

Get tips on using pMCY87 to perform Protein Expression Prokaryotic cells - E. coli PduP-E bacteriophage ϕX174

Get tips on using pMCY86 to perform Protein Expression Prokaryotic cells - E. coli PduP-E bacteriophage ϕX174

Get tips on using pMCY85 to perform Protein Expression Prokaryotic cells - E. coli PduP-E bacteriophage ϕX174

Get tips on using pGB2B3 to perform Protein Expression Prokaryotic cells - E. coli hβ-defensin 2/3