Protein expression and purification Mammalian cells HEK 293

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pET-32c Product

Get tips on using pET-32c to perform Protein Expression Prokaryotic cells - E. coli rpf-like protein

Products R Verma, Bacteriology and Mycology Division, Mycobacteria Labora pET-32c

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Cells Immortalized cell lines SH-SY5Y

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary porcine primary airway epithelial cell

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary rat dorsal root ganglion neurons

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary mouse dorsal root ganglion neurons
pIL-15 Product

Get tips on using pIL-15 to perform Protein Expression Eukaryotic cells - HEK293 IL-15

Products JianweiZhu, Engineering Research Center of Cell & Therapeutic An pIL-15

Get tips on using pFastBac1-TEV-TRPV1 to perform Protein Expression Eukaryotic cells - HEK293 TRPV1

Products Yifan Cheng, Department of Biochemistry and Biophysics, Keck Adv pFastBac1-TEV-TRPV1

Get tips on using pCMV-HA2/DKK1 to perform Protein Expression Eukaryotic cells - HEK293 DKK1

Products G.Y. Bao, Department of Clinical Laboratory, Yangzhou First Peop pCMV-HA2/DKK1

Get tips on using pgMAX system-DsRed2 to perform Protein Expression Eukaryotic cells - HEK293 DsRed2

Products Manabu Murakami, Department of Pharmacology, Hirosaki University pgMAX system-DsRed2
pCEP-4-MBP Product

Get tips on using pCEP-4-MBP to perform Protein Expression Eukaryotic cells - HEK293 BM40

Products Manuel Koch, Institute for Dental Research and Oral Musculoskele pCEP-4-MBP

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