The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Get tips on using EasySep™ Human B Cell Enrichment Kit to perform Cell Isolation B cell
Get tips on using RosetteSep™ Human B Cell Enrichment Cocktail to perform Cell Isolation B cell
Get tips on using EasySep™ Human B Cell Isolation Kit to perform Cell Isolation B cell
Get tips on using StemSep™ Human CD34 Positive Selection Cocktail to perform Cell Isolation CD34+ cells
Get tips on using Human/Mouse/Rat/Canine ALCAM/CD166 Antibody to perform Immunohistochemistry Mouse - CD166 / ALCAM
Get tips on using Brilliant Violet 510™ anti-human HLA-DR Antibody to perform Flow cytometry Anti-bodies Human - HLA-DR
Get tips on using PerCP-Cy™5.5 Mouse Anti-Human HLA-DR to perform Flow cytometry Anti-bodies Human - HLA-DR
Get tips on using PE/Dazzle™ 594 anti-human CD184 (CXCR4) Antibody to perform Flow cytometry Anti-bodies Human - CD184/CXCR4
A standard angiogenic assay involves the autonomous endothelial cell response of self-organization into microvessels, also known as tubes when seeded on a basement membrane matrix in the presence of the appropriate growth factors. However, the component of basement membrane matrix may also affect the tube formation by endothelial cells. Hence it is important to use a standard angiogenesis assay kit or use the same membrane matrix with known composition to standardize the assay conditions.
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment