Site Directed Mutagenesis (SDM) Rat Point mutation Rat-2

Get tips on using Cytochrome c Profiling ELISA Kit (ab110172) to perform ELISA Rat - Cytochrome c

Get tips on using Ion Total RNA-Seq Kit v2 to perform RNA sequencing Rat - PC12

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Rat - PC12

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Rat - A7R5

Get tips on using Anti-Glutamine Synthetase Antibody, clone GS-6 to perform Immunohistochemistry Rat - GS

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Rat - Heart

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Rat - H4IIE

Get tips on using Magna ChIP™ A - Chromatin Immunoprecipitation Kit to perform ChIP Rat - Liver

Get tips on using Magna ChIP™ A - Chromatin Immunoprecipitation Kit to perform ChIP Rat - Brain

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.