Cell cycle assay mouse

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Purified anti-mouse TNF-α Antibody to perform Flow cytometry Anti-bodies Mouse - TNF-α

Get tips on using FITC anti-mouse CD95 (Fas) Antibody to perform Flow cytometry Anti-bodies Mouse - CD95/Fas

Get tips on using APC anti-mouse CD252 (OX40L) Antibody to perform Flow cytometry Anti-bodies Mouse - CD252/OX40L

Get tips on using Purified Rat Anti-Mouse IFN-γ to perform Flow cytometry Anti-bodies Mouse - IFN-γ

Get tips on using APC Rat Anti-Mouse IFN-γ to perform Flow cytometry Anti-bodies Mouse - IFN-γ

Get tips on using Purified Rat Anti-Mouse Siglec-F to perform Flow cytometry Anti-bodies Mouse - Siglec F

Get tips on using BV421 Rat Anti-Mouse Siglec-F to perform Flow cytometry Anti-bodies Mouse - Siglec F

Get tips on using siGENOME Mouse Amotl2 (56332) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - MS1 AmotL2

Get tips on using siGENOME Mouse Pard3 (93742) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - MS1 Pard3